Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Binary plasmid containing aroA in both sense (octopine synthetase or ocs promoter) and anti-sense (mas promoter) orientations. The presence of the anti-sense construct is effective in reducing aroA expression relative to controls containing the gene only in the sense orientation. USDA permit (APHIS form 2000) required for distribution. (ATCC staff) Restriction digests of the clone give the following sizes (kb): SalI--21.0, 8.8, 6.0; BglII--20.2; 15.0; EcoRI--22.0, 4.4, 4.0, 3.4, 1.6; BamHI--25.6, 8.0, 1.6; ClaI--22.0, 6.5, 2.6, 2.4, 1.8. (ATCC staff) Medium is -1 1065 plus kanamycin (50 ug/ml) plus streptomcyin (20 ug/ml). Hosts: Agrobacterium tumefaciens K12, Agrobacterium tumefaciens, E.coli, higher plants. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number