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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCLL9

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9910
Notes
Restriction digests of the clone give the following sizes (kb): BamHI--10.2; BglII--10.2; PstI--10.2. (ATCC staff) Cloning into the PstI site inactivates the ampicillin marker used for selection in Escherichia coli. Cloning into the BglII, SphI, or SstI sites inactivates the tyrosinase gene. The pAkij1 ori may be useful in other taxa such as Bacillus, Staphylococcus and other Actinomycetes, including Streptosporangium, Actinoplanes, Nocardia, and Micromonospora. [1] Note: This material is cited in a U.S and/or other Patent and may not be used to infringe the patent claims. Growth: LB plus ampicillin (ATCC medium number 1227) 37C Hosts: E.coli, E.coli W5445, Actinomadura kijaniata, Streptomyces lividans. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
67449
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified