Vector Database

Derived from the ligation product of a 7.6 kb PstI fragment of pPS10 and a 4.2 kb PstI fragment of pKT231 which was then shortened by deletion. A 1.8 kb region of pPS10 was retained (EMBL accession number X58896) which encodes replication functions. Compatible with broad-host-range plasmids of the incompatibility groups P1, P4/Q and W, and maintained at a copy number of 15-20 in P. savastanoi. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.95; HindIII--4.2; BamHI/KpnI--2.2, 2.0. (ATCC staff) The order of the major features in this plasmid is:PvuII - BamHI - kanR - HindIII - KpnI - repA. Medium is 1236 LB plus kanamycin. Hosts: Pseudomonas aeruginosa PAO1024 [ATCC 47053], Pseudomonas, P.aeruginosa PAO1162, P.putida KT2440 [ATCC 47054]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)