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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCN41

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4500
Notes
Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 & 1500), with the site at nt 536 between the repA promoter and coding sequences. (personal communication) General purpose vector. (ATCC staff) Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut. (ATCC staff) Medium is -1 1065 plus streptomycin. Hosts: Pseudomonas aeruginosa PAO1024 [ATCC 47053], Pseudomonas, P.aeruginosa PAO1162, P.putida KT2440 [ATCC 47054]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77098
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified