Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Restriction digests of the clone give the following sizes (kb): PstI--14, 4.3; AccI--3.3, 2.7, 2.7, 2.5, 2.5, 2.2, 1.5, 1.3; BglI--11.0, 4.5, 2.5; EcoRI--4.2, 3.3, 2.9, 2.9, 1.7, 1.3, 1.3. (ATCC staff) The presence of the POT1 gene ensures stable maintenance of the plasmid in yeast during growth on nonselective medium using glucose as a carbon source.  An episomal Saccharomyces cerevisiae/E.coli shuttle expression vector containing 2 micron circle, pBR322 replication and selection, and Schizosaccharomyces pombe sequences encoding triose phosphate isomerase (POT1).  Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number