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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pCS19

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
9344
Notes
Shuttle vector for construction of genomic libraries that permits direct selection of recombinants. Insertion into the BclI site inactivates the cI repressor, allowing expression of tetracycline resistance in recombinants. Propagation in a dam- host is necessary for cleavage at the BclI site. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46; HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59; SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75. The order of the major features in the plasmid is: EcoRI - ARS1 - SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR - trp terminator - BamHI - pMB1 ori - ampR. [2] Growth medium: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: C.G.Sengstag Hosts: E.coli, Saccharomyces. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77409
GenBank
M98046
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence