Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCYM00
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9408
- Notes
- Constructed by inserting a BamHI/SalI fragment from pMC1403 (lac operon beginning at codon 9 of lacZ) between the BamHI/BalI (SalI-linkered) sites of pCYM0. Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and mouse cells, and thus can be used as a control to calibrate transformation efficiency. Restriction digests of the clone give the following sizes (kb): BamHI/SalI--6.0(doublet); EcoRI--12.0; SmaI--12.0; Pvu/BamHI--6.5, 2.5, 2.1, 0.54, 0.49. (ATCC staff) Our plasmid is 12 kb instead of 9.4, which matches the original depositor's DNA. (ATCC staff) Shuttle reporter plasmid. Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37821
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified