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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Constructed by deleting the HpaI (1686)/SmaI (2349) fragment from pCMY0 (ATCC 37820), removing part of the SV40 terminator region from pSV2-cat & part of the URA3-associated sequence from pUT322. The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; BamHI/PvuII--4.9, 0.5. (ATCC staff) Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number