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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter. Shuttle expression vector. (ATCC staff) Permits expression in both S. cerevisiae and mouse cells. (ATCC staff) Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing URA3d into pCYM2 cut with BglII & ClaI (nt 3469 and 3589 according to the pCYM0 numbering scheme). URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation. Restriction digests of the clone give the following sizes (kb): BglII--uncut; ClaI/BamHI--3.4,2.8,0.5; EcoRI--6.6; BamHI/PvuII--6.0, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli CM5alpha, Saccharomyces cerevisiae, vertebrate cells, E.coli, Saccharomyces cerevisiae W303-1B/D, mouse C11D cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number