Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Shuttle vector permitting positive selection in E. coli. Cloning sites are positioned in the EcoRI endonuclease gene, regulated by a lambda promoter. In E. coli hosts providing the temperature-sensitive lambda repressor cI857, growth at 37C will occur only if the endonuclease gene is interrupted. The non-enzymatic chloramphenicol resistance gene, derived from Rhodococcus rhodochrous SC2318 (ATCC 12674), does not confer resistance in E. coli. The plasmid confers immunity to nocardiophage Q4.  Growth media: LB plus ampicillin (ATCC medium number 1227) 30C Deposited by: E.R.Dabbs Hosts: Rhodococcus, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number