Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pDdGal-16
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8500
- Notes
- pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (ATCC 37772) differ in their polylinker sequences. Each contains a BglII site in a different reading frame relative to the lacZ gene. A promoter-cloning plasmid vector using expression of beta-galactosidase as the reporter in Dictyostelium. The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were inserted into a derivative of pB10TP2 from which the f1 origin and and a lacZ fragment had been removed. The neo gene is regulated by an actin 6 promoter and a CP1 terminator. Restriction digests of the clone give the following sizes (kb): EcoRI--8.5; BamHI--8.5; BglII--8.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Dictyostelium discoideum, E.coli SURE, E.coli DH5. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37771
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified