Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pDG3
| Source/Vendor: | ATCC |
| Plasmid Type: | Unspecified |
| Cloning Method: | Unknown |
| Size: | 7800 |
| Notes: | Restriction digests of the clone give the following sizes (kb): HindIII/SalI--3.1, 1.65, 1.3, 0.75, 0.5 + smaller; XbaI--4.4, 3.4; EcoRI--7.8; SmaI--4.3, 3.5; SphI--7.8. (ATCC staff) This was constructed by inserting a HindIII/SalI fragment of Tn5 into pUC12 cut with HindIII/SalI (pNE02) and then inserting an XbaI fragment from pBB116 into the XbaI site of pNE02. [1] This is an integrative, selectable vector conferring arginine prototrophy on arginine autotrophic hosts such as Aspergillus niger 350(-)52, ATCC 20739 (argB-). It also confers kanamycin resistance.[1] Medium is 1227 LB plus ampicillin. Hosts: E.coli JM83, Aspergillus niger, E.coli, Aspergillus niger 350-52 [ATCC 20739]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
| Catalog Number: | 53006 |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Unspecified |