Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- NCRR YRC
- Plasmid Type
- Cloning Method
- Plasmid pDH5 contains the ORF for a pH-tolerant yellow-fluorescent protein (YFP), a variant of GFP. The plasmid also contains the selectable marker from S. pombe that complements S. cerevisiae his3. The codons have not been optimized for mammalian expression; instead the codons are the same as in the parent vector (except for the 5 mutations listed below). Mutations in the GFP(S65T) ORF: S65G, V68L, Q69K, S72A, T203Y. Parent vector: pFA6a-GFP(S65T)-HIS. See Wach et al. 1997.