Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pDK101
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3027
- Notes
- Deposited by: Bernard Weisblum Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. (ATCC staff) Vector useful for cloning-double stranded amplification products. Permits synthesis of ssDNA and contains promoters for in vitro RNA synthesis. [1] The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. [1] The order of the major features in this plasmid is: T7/ApaI/MCS/NsiI/lacZ'SP6 - pMB1 ori - pMB1 - ampR - f1 ori. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli JM109. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77406
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified