Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
|Alt Name:||pDONR 223|
|Plasmid Type:||Other, Gateway Donor vector|
|Cloning Method:||Gateway Cloning|
|5' Sequencing 1 Primer:||M13F/M13R|
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors. The sequences of the attP1 and attP2 sites in pDONR223 are identical to those in the original pDONR201 Entry vector. Reference: Rual et al, Genome Res. 14:2128-2135, 2004. Requires ccdB resistant strain of bacteria.