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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pDUAL/pJANUS

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
7900
Notes
Cosmid cloning vector for generating nested deletions and sequencing templates in both directions in vivo. Based on the transposon gamma-delta (Tn1000), and contains paired selectable and counterselectable markers. Deletions of a pDUAL clone can be obtained by transforming into a host expressing the gamma-delta transposase, such as E. coli CBK884(pXRD4043) (ATCC 47073), followed by antibiotic selection and sensitivity screening. Deletion derivatives may be sequenced from the SP6 or T7 promoters engineered within the ends of the gamma-delta component. The order of the major features is: RsrII - SP6 promoter - Tn end - sacB (sucrose sensitivity) - kanR - (lacZalpha - EcoRI/MCS/HindIII - lacZalpha) - tetR - cos - rpsL/strA (strS) - Tn end - T7 promoter - NotI - pMB1 ori - Tn1000 - ampR. [1] Deposited by: C.M.Berg Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Hosts: E.coli. Related vectors: pXRD4043, pACYC184. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) More information: http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=86961
Catalog Number
86961
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified