Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pET-21 c (+)
|Alt Name:||pET21c, pET-21 c, pET-21c, pET21c(+), pET-21 c(+), pET-21c(+)|
|Plasmid Type:||Bacterial Expression|
|Cloning Method:||Restriction Enzyme|
|5' Sequencing 1 Primer:||T7|
|5' Sequencing 1 Primer Sequence:||5'-TAATACGACTCACTATAGGG-3'|
|3' Sequencing 1 Primer:||T7-term|
|3' Sequencing 1 Primer Sequence:||5'-GCTAGTTATTGCTCAGCGG-3'|
|Tag 1:||T7-tag (N-term)|
|Tag 2:||His-tag (C-term)|
pET-21c(+) is a bacterial expression vector that contains an N-terminal T7-Tag and an optional C-terminal His-Tag. pET-21c(+) and pET-24c(+) differ by bacterial resistance. pET-21a,b,d differ by the length of the MCS, for cloning in-frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.