Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pET-21 c (+)
| Source/Vendor: | EMD Biosciences |
| Alt Name: | pET21c, pET-21 c, pET-21c, pET21c(+), pET-21 c(+), pET-21c(+) |
| Analyze: | Sequence |
| Plasmid Type: | Bacterial Expression |
| Promotor: | T7 |
| Expression Level: | High |
| Cloning Method: | Restriction Enzyme |
| Size: | 5441 |
| 5' Sequencing 1 Primer: | T7 |
| 5' Sequencing 1 Primer Sequence: | 5'-TAATACGACTCACTATAGGG-3' |
| 3' Sequencing 1 Primer: | T7-term |
| 3' Sequencing 1 Primer Sequence: | 5'-GCTAGTTATTGCTCAGCGG-3' |
| Tag 1: | T7-tag (N-term) |
| Tag 2: | His-tag (C-term) |
| Bacterial Resistance: | Ampicillin |
| Notes: | pET-21c(+) is a bacterial expression vector that contains an N-terminal T7-Tag and an optional C-terminal
His-Tag.
pET-21c(+) and pET-24c(+) differ by bacterial resistance. pET-21a,b,d differ by the length of the MCS, for cloning in-frame.
The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. |
| Catalog Number: | 69742-3 |
| Stable: | Transient |
| Constitutive: | Constitutive |
| Viral/Non-Viral: | Nonviral |