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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pET-21 c (+)

Source/Vendor: EMD Biosciences
Alt Name: pET21c, pET-21 c, pET-21c, pET21c(+), pET-21 c(+), pET-21c(+)
Analyze: Sequence
Plasmid Type: Bacterial Expression
Promotor: T7
Expression Level: High
Cloning Method: Restriction Enzyme
Size: 5441
5' Sequencing 1 Primer: T7
5' Sequencing 1 Primer Sequence: 5'-TAATACGACTCACTATAGGG-3'
3' Sequencing 1 Primer: T7-term
3' Sequencing 1 Primer Sequence: 5'-GCTAGTTATTGCTCAGCGG-3'
Tag 1: T7-tag (N-term)
Tag 2: His-tag (C-term)
Bacterial Resistance: Ampicillin
Notes:
pET-21c(+) is a bacterial expression vector that contains an N-terminal T7-Tag and an optional C-terminal His-Tag. pET-21c(+) and pET-24c(+) differ by bacterial resistance. pET-21a,b,d differ by the length of the MCS, for cloning in-frame. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
Catalog Number: 69742-3
Stable: Transient
Constitutive: Constitutive
Viral/Non-Viral: Nonviral

Generated Plasmid Map

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