Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pEX13
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5813
- Notes
- Created by Moore, July 1995, under contract with NCBI. This strain is resistant to 100 ug/ml ampicillin. (personal communication) This is one of three bacterial expression vectors (pEX11, ATCC 37708; pEX12, ATCC 37709; and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda. [2] This was constructed from pEX3 by ligating a synthetic oligonucleotide into pEX3 cut with XmaI and BamHI. The oligonucleotide added several unique restriction sites. [1] The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BamHI--5.8; PstI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli POP2136 [ATCC 47049], E.coli. Related vectors: pEX3. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37710
- GenBank
- X03174
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified