Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Deposited by: Klaus Geider Suicide plasmid for transposon-mediated or site-directed mutagenesis of Erwinia amylovora and Escherichia coli after electroporation.  Suggested conditions for electroporation in these bacterial strains: E. coli C600Sm, fields strength 20 kV/cm, pulse length 5 to 12 msec; Erwinia amylovora Ea7/74Sm, 25 kV/cm, 5 msec; E. amylovora Ea1/79Sm, 12.5 kV/cm, 17.5 msec.  To propagate the plasmid, the host must provide the bacteriophage fd gene 2 product.  The order of the major features in this plasmid is: EcoRI - ampR - fd ori - EcoRI - Tn5/kanR (6.5 kb).  Restriction digests of the clone give the following sizes (kb): EcoRI--6.5, 2.0; BamHI--4.4, 4.0; HindIII--5.0, 3.4; PstI--2.45 (doublet); 1.5, 0.96, 0.84. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli C600-2Sm. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)