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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pFN476

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4200
Notes
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1. (ATCC staff) The order of the major features of the plasmid is:pSC101 rep > - BamHI - < 3' lacZ - T7 promoter > - EcoRI/MCS(pGEM-3Z)/HindIII - < SP6 promoter - 5' lacZ - BamHI - tnpR > - ampR > - par - pSC101 ori. (personal communication) Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects. Maintained at 1-5 copies per cell. At this level, the effect of uninduced expression of T7 RNA polymerase from the UV5 promoter resulting in transcripts of potentially toxic inserts is minimized. T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303). [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Sankar P.M. Hosts: E.coli, E.coli RC104. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
86962
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified