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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pGCAT-C

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 4552
Restriction digests of the clone give the following sizes (kb): PvuII--3.0, 1.5; HincII--3.0, 1.5; EcoRI--4.25, 0.3. (ATCC staff) The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The sequence at the junction of the multiple cloning site and CAT is GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT. (personal communication) BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII sites were filled in. Contains SV40 polyadenylation site and donor and acceptor sites of SV40 small-t intron. A plasmid shuttle vector for investigating promoter and/or enhancer elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7 promoters for in vitro RNA synthesis and low basal CAT expression in eukaryotic cells. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. (Information source: VectorDB ( ).)
Catalog Number: 37641
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified