Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
|Alt Name:||pGEM3, pGEM3Zf|
|Plasmid Type:||Bacterial Expression|
|Promotor:||T7 and SP6|
|Cloning Method:||Restriction Enzyme|
|5' Sequencing 1 Primer:||T7|
|3' Sequencing 2 Primer:||SP6|
This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The sequence is the strand that is secreted by cells harboring pGEM-3Zf(-) plasmids. Hosts: E.coli JM109. Related vectors: pUC18, pGEM-3Z, pGEM-3Zf+. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)