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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pGEM-3Zf-

Source/Vendor: Promega
Alt Name: pGEM3, pGEM3Zf
Analyze: Sequence
Plasmid Type: Bacterial Expression
Promotor: T7 and SP6
Expression Level: High
Cloning Method: Restriction Enzyme
Size: 3199
5' Sequencing 1 Primer: T7
3' Sequencing 2 Primer: SP6
Bacterial Resistance: Ampicillin
This vector can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region. The sequence is the strand that is secreted by cells harboring pGEM-3Zf(-) plasmids. Hosts: E.coli JM109. Related vectors: pUC18, pGEM-3Z, pGEM-3Zf+. (Information source: <a href= target=_blank>VectorDB</a>.)
GenBank: X65307
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Nonviral

Generated Plasmid Map