Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pGEX-KT
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4978
- Notes
- Deposited by: David J. Hakes, Jack E. Dixon The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. [1] Expression vector for rapid purification of fusion proteins and release of proteins with fewer extraneous N-terminal amino acids. [1] The resulting protein contains two additional amino acids at the amino terminus (Gly-Ser). [1] Constructed from pGEX-1 by inserting an oligonucleotide at the BamHI site which encodes the glycine kinker and thrombin recognition site to enhance cleavage of the fusion protein. [1] The order of the major features in the plasmid is: pMB1 ori - lacIq - lacZ - tac promoter - GST - glycine kinker - thrombin cleavage site - BamHI/MCS/EcoRI - ampR. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI/PstI--4.0, 1.0; EcoRI/EcoRV--3.2, 1.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM101, E.coli TG1, E.coli BL21. Related vectors: pGEX-1, pGEX-KN, pGEX-KG. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
- Catalog Number
- 77331
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified