Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pHP45omega-Km

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4500
Notes
[2] describes a procedure combining the advantages of in vitro DNA linker mutagenesis with those of in vivo transposition mutagenesis. Insert the symmetrical 2.0-kb omega fragment, with an antibiotic resistance gene flanked by short inverted repeats carrying translation termination signals and synthetic polylinkers, into a linearized plasmid. The omega fragment terminates RNA and protein synthesis prematurely, allowing the definition and mapping of both transcription and translation units. Because of its symmetry, it can be inserted in either direction. Cut pHP45omega with BssHII + SphI, removing the spcR/strR gene, and make the ends blunt. Ligate this to a blunt-end Tn5 1.5-kb HindIII-SalI fragment, containing the kanamycin resistance gene. [1] Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate. [1] This is one of a series (ATCC37653-ATCC37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative bacteria. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--2.35, 2.35; HindIII--2.4, 2.3; SalI--4.7. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 209010 Hosts: E.coli C600, broad host range, E.coli. Related vectors: pBR322, pKTH604, R100.1 omega element, pHP45omega-Cm, pHP45omega-Hg, pHP45omega-Tc. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37653
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified