Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pHPS9R
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4100
- Notes
- Recommended for pHPS9 (ATCC 37816, 37817) to permit alpha-complementation and plasmid marker rescue transformation for transformation of competent cells with plasmid monomers. pHPS9R serves as the helper plasmid. Differs from pHPS9 (ATCC 37816, 37817) by partial removal of chloramphenicol resistance sequence (downstream of the NcoI site) and removal of the P59 promoter and sequences encoding the cat86::lacZalpha fusion. Plasmid was not verified by restriction analysis. Strain was checked for the following phenotypes: showed growth on LB + erythromycin (150 ug/ml), white colonies on Xgal +IPTG, white colonies on Xgal + IPTG + erythromycin (150 ug/ml). (ATCC staff) Medium is -1 1065 plus erythromycin (150 ug/ml). Hosts: Bacillus subtilis 6GM15, Bacillus subtilis, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37818
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified