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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pHY300PLK

Information

Source/Vendor
TaKaRa
Plasmid Type
Bacterial Expression
Cloning Method
Unknown
Size
4870
Bacterial Resistance
ampicillin and tetracycline
Notes
PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. Twenty-three unique recognition restriction endonuclease sites have been found in pHY300PLK. Hosts: E.coli, B.subtilis, E.faecalis strain DS-5, S.faecalis. Related vectors: pACYC177, pAMalpha, pHY320PLK. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) Escherichia coli-Bacillus subtilis shuttle vector with ampicillin and tetracycline resistance genes.
GenBank
D00054
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence