Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pIA2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9300
- Notes
- YE-type shuttle vector permitting FLP-driven high copy number. (personal communication) In S. cerevisiae, requires a host expressing FLP such as YPH485 or YPH494, or a host modified by pFV17 (ATCC 77170). (personal communication) When propagated in a strain carrying an integrated copy of GAL10-FLP, the copy number is 10-20 without induction and 200-400 when induced by galactose. The order of the major features in this plasmid is:REP3 - D - REP1 - REP2 - TRP1 - MCS (NotI - BamHI/NotI) - kanR. Constructed by inserting a BalI/EcoRV fragment of the 2 micron circle (interrupting FLP) into the SmaI site of pHSS6 which had been modified by the addition of a TRP1 gene. Restriction digests of the clone give the following sizes (kb): HindIII--4.8,2.2,1.4, 1.2; BamHI--9.6; NotI--9.6; ClaI--9.6. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH485, Saccharomyces cerevisiae YPH494. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77160
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified