Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pJAK16

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
12600
Notes
The order of the major features in this plasmid is:tac promoter - EcoRI/MCS/HindIII - rrnB terminator - cmlR - oriV - oriT - RSF1010 ori - lacIq. (submitted, 1992) Restriction digests of the clone give the following sizes (kb): EcoRI--12.6; HindIII--12.6; SphI--9.0, 3.6. (ATCC staff) A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. (submitted, 1992) Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. [1] Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. (submitted, 1992) Compatible with common E.coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. (submitted, 1992) Medium is 1675 LB plus chloramphenicol (10 ug/ml). Hosts: E.coli SCS1, broad host range, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77291
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified