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Addgene

Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pJD220SVHy

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5100
Notes
pJD214Hy was constructed from 1.45 kb of SNV DNA ligated into the BamHI/EcoRI (nt 2066 to nt 4361) fragment of pBR322, a pUC12 polylinker, and the hygromycin phosphotransferase B gene (BamHI fragment blunt end-ligated into the HindIII site of the MCS). [2] An intermediate, pJD217SVHy, was constructed from pJD214Hy by deleting a SacI/AvaI fragment (SNV map units 7.747-8.127) and replacing it with an XhoI linker, and inserting a 565 bp NdeI/HindIII fragment from pSV2-neo (SV40 promoter) at the XbaI site. [3] Constructed from pJD217SVHy by inserting a 220 bp BamHI/HindIII fragment (SV40 poly(A) site) into the BamHI site at the 3' end of U5. [3] A replication-defective retroviral vector which is promoter-deficient in the right LTR and cannot express retroviral RNA. Foreign gene expression is regulated by the SV40 early promoter. [3] Removal of SNV map units 7.747 to 8.127 results in deletion of the entire U3 sequence, except for 10 bp at the 5' end containing the attL site. [1] The 5' 10 bp of U3 in the left LTR were deleted, so there is no homology between the two U3 sequences and the risk of recombination is reduced. [1] pJD220SvHy (ATCC 67393) and pRD8 (ATCC 67394) differ in the orientation of the SV40 promoter/hygromycin phosphotransferase B sequences. pRD8 has an additional 3' RNA processing sequence between the left U5 and the hygromycin sequences. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 0.4; PstI--2.7, 2.3, 0.4; XbaI--5.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, vertebrate cells, D17-C3 helper cell line for transfection, D17 cell line for infection. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
67393
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified