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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pJQ210SK

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 7200
Notes:
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - cos - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 77489
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified