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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pKO-2

Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 3755
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pKO1 and according to reference 1 by F. Pfeiffer. This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galK gene. Also, a polylinker was cloned before this gene. pKO2 contains a 4-bp deletion not present in pKM2. However, in GenBank SYNPKO2 and SYNPKM2 have been mixed up. Thus, SYNPKM2 is given in the Parent section. Hosts: E.coli GALK-, bacteria GalE-T-K-. Related vectors: pKO-1, EcoGalK, pBR322, pKM2. (Information source: VectorDB ( ).)
GenBank: L08924
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map