Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pKO-2
Analyze: | Sequence |
Plasmid Type: | Unspecified |
Cloning Method: | Unknown |
Size: | 3755 |
Notes: | These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from pKO1 and according to reference 1 by F. Pfeiffer. This is a promoter probe vector. The promoterless galK gene is used as selectable marker. Translationsal stop codons in all reading frames are present on a 11-bp oligonucleotide before the galK gene. Also, a polylinker was cloned before this gene. pKO2 contains a 4-bp deletion not present in pKM2. However, in GenBank SYNPKO2 and SYNPKM2 have been mixed up. Thus, SYNPKM2 is given in the Parent section. Hosts: E.coli GALK-, bacteria GalE-T-K-. Related vectors: pKO-1, EcoGalK, pBR322, pKM2. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
GenBank: | L08924 |
Stable: | Unspecified |
Constitutive: | Unspecified |
Viral/Non-Viral: | Unspecified |