Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pLA2917
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 21000
- Notes
- A broad host range plasmid vector. This is a cosmid with a broad host range that can be used to characterize the genome of C1-utilizing microorganisms. [2] This cosmid can be mobilized for transfer by conjugation between E.coli and other strains in the presence of helper plasmid pRK2013. [2] The BglII and PstI sites interrupt the kanamycin resistance gene; the SalI site interrupts the tetracycline resistance gene. [2] This vector transfers efficiently to a broad host range including E.coli HB101, Methylobacterium organophilum DSM 761, Pseudomonas AM1, Pseudomonas putida, and Pseudomonas aeruginosa. [2] DNA inserts, 25-40 kb in size, can be cloned into the BglII site and mobilized from E.coli to M. organophilum.(personal communication) Restriction digests of the clone give the following sizes (kb): PstI--21.0; HindIII--21.0; BglII--21.0; EcoRI--17.0, 4.0; (ATCC staff) Medium is 1273 LB plus tetracycline. Hosts: E.coli HB101, broad host range, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 39840
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified