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Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pM5 neo


Plasmid Type
Mammalian Expression
Cloning Method
Bacterial Resistance
Selectable Marker
Laker et al. Proc Natl Acad Sci USA Vol 84, p 8458. M5-neo was constructed by subcloning several fragments derived from the original MPSV-containing plasmid and the Tn5 gene and subsequent ligation by way of polylinkers. Briefly, a PstI fragment containing the 5'LTR and 5' splice signal, a HindIII-SmaI fragment containing the 3' splice signal, and a HindIII-BglII fragment containing the 3'LTR from MPSV, as well as a SmaI-SalI fragment with the neo resistance coding region of Tn5, were ligated in the BglII-HindIII site of pBR322 (minus EcoRI site).