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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pMA200U

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4152
Notes
Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the analogous 1.27 kb fragment of pTZ18U. Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain containing a pMA200U derivative produces packaged single-stranded plasmid DNA complementary to the M13 universal primer. If the insert is oriented correctly for transcription from the lambda promoters, the single-stranded plasmid DNA will contain the coding strand. Restriction digests of the clone give the following sizes (kb): BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3. (ATCC staff) Expression vector encoding cI857. Contains primer sites useful for sequencing and permits production of ssDNA. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli AN1459, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37833
GenBank
M36426
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified