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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pMAC5-8

Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 3803
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Corrected 20-DEC-1986 by J. Thierack, MPI Biochemie, Martinsried Corrected 10-JUL-1987 by P. Stanssens and F. Pfeiffer Vector for multiple rounds of oligonucleotide-directed construction of mutations. One derivative (pMA5) confers ampicillin resistance, the other (pMC5) confers resistance to chloramphenicol. Using specific oligonucleotides, resistance is shifted together with site-directed mutagenesis between these two resistance modes. The PstI site in the polylinker region is not unique. Presence of CAA at pos 2811-13 was shown by DNA sequencing. It is unknown, if this also applies to the parent sequence (pBR325)! Hosts: E.coli. Related vectors: PF1, pBR325, Poly8, EcoTrp. (Information source: VectorDB ( ).)
GenBank: L08930, A10238, A06419, A08598
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map