Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMMB206
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9311
- Notes
- Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction. [1] Sequencing demonstrated that there are 3 stop codons in frame with the truncated bla gene, terminating translation but permitting continued transcription and expression of the cat gene. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--9.3; EcoRV--5.8, 3.6; SspI--3.9, 1.8, 1.6, 1.2, 0.9; EcoRI/MluI--8.4, 0.9. (ATCC staff) Medium is 1675 LB plus chloramphenicol (10 ug/ml). Hosts: E.coli TB1, E.coli DH5alphaF', E.coli, Gram-positive bacteria, broad host range. Related vectors: pMMB190, pMMB66EH. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37808
- GenBank
- M37847, M28994
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified