Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMSE
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5400
- Notes
- Kanamycin resistance should be checked at regular intervals. This is best done during amplification steps using LB medium supplemented with 50 ug/ml ampicillin and 12.5 ug/ml kanamycin. (personal communication) The SV40 promoter has a 3 bp deletion at the BglI site within the origin of replication to prevent autonomous replication in the presence of SV40 large T-antigen. The MSV LTR provides a polyadenylation signal as well as an enhancer for the P1/thymidine kinase promoter regulating the Tn5 kanR/G418R transcription unit. A BamHI/HindIII digest gives fragments of 3.0 and 2.5 kb. Restriction digest of the clone give the following sizes (kb): HindIII--5.9; BamHI--5.9; EcoRI--5.9; BamHI/HindIII--3.2, 2.7; BglII--5.9; consistent with deposited DNA. (ATCC staff) The order of the major features in this plasmid is:SV40 promoter - MSV-LTR - P1 promoter - TK promoter - kanR/G418R - TK polyadenylation signal - ampR. Shuttle expression vector. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pUC19, pHS272, pMSPE. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37834
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified