Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pMX-EGFP-gw-IP
| Plasmid Type: | Mammalian Expression, Retroviral |
| Cloning Method: | Unknown |
| 5' Sequencing 1 Primer: | pBMN5' |
| Bacterial Resistance: | Ampicillin |
| Selectable Marker: | Puromycin |
| Notes: | For constructing pMX-EGFP-gw-IP, a gateway cassette rfB (Invitrogen) was introduced into XhoI (blunted) site of pEGFP-C2 (Clontech). We named this product, pEGFP-gw.
Second, an EcoRV/XbaI (blunted) fragment of pIRESpuro (Clontech) was introduced into BamHI (blunted)/SalI (blunted) site of pMX.
Finally, an Eco47III (blunted)/HindIII (blunted) fragment of pEGFP-gw was introduced into the EcoRI (blunted)/BamHI (blunted) of pMX-IP vector. This product is pMX-EGFP-gw.
Information provided by Dr. Kazutoshi Takahashi and Dr. Shinya Yamanaka. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Retroviral |