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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pNH218

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
11500
Notes
Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 3.8; HindIII--6.8, 4.7. (ATCC staff) A 1.1 kb EcoRI fragment with Bacillus licheniformis alpha-amylase regulatory & signal sequences was cloned into pUC9 cut with EcoRI/SmaI. A 3.0 kb BamHI/HindIII fragment (E.coli phoA minus signal sequences and the first 5-13 aa) was inserted. pBs81/6 (Bacillus replication) was cut with HindIII and cloned into the HindIII site. An E.coli/Bacillus spp. shuttle expression vector for the construction and secretion of fusion proteins. Contains the alkaline phosphatase gene of E.coli. The plasmid appears to be unstable. We recommend that the ATCC-provided stock be streaked for single colonies and that a colony with the correct restriction pattern be selected. (ATCC staff) Medium is 1065 LB medium. Hosts: Bacillus subtilis 168, Bacillus subtilis, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
53063
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified