Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pPLa8
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 3716
- Notes
- The following unique restriction sites are found on this vector separated by (bp)(approx): BamHI - 900 - EcoRI - 600 - XhoI - 300 - SmaI - 250 - HindIII - 1650. [1] Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--3.0, 0.7; BglI/XhoI--2.2, 1.5; SmaI--3.7; ClaI--3.7; HindIII--3.7. (ATCC staff) This vector was derived from pPLa2311 (ATCC 31694) by using linkers to convert the PstI site in the beta-lactamase gene to a BamHI site. The Ala at position 182 is replaced with Arg-Ile-Arg & beta-lactamase activity is lost. [1] The orientation of the PL promoter is anticlockwise with respect to the plasmid ori. [1] E.coli K-12 deltaH1 is K-12 M72 lac(am) deltatrpEA2 rpsL lambda cI857 N(am7)N(am53) deltaH1 bio-. deltaH1 removes part of cro & all genes to the right of cro. [1] This vector is used for expression of fragments containing a ribosome binding site. [1] Medium is 1236 LB plus kanamycin. Hosts: E.coli deltaH1, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 31695
- GenBank
- M17233
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified