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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pQF60

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5800
Notes
Promoter-cloning shuttle vector permitting visual detection of activity. Uses the promoterless alkaline phosphatase gene (phoA) as the reporter group. The order of the major features in this plasmid is: ori(pMB1) - ori(1600) - ampR - terminator - multiple cloning site - phoA. Translational stop codons in all reading frames are located between the MCS and the translational initiation codon of the reporter gene. Restriction digests of the clone give the following sizes (kb): HindIII--5.8; BamHI--5.8; EcoRI--4.6, 1.2; XbaI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM109, broad host range, E.coli, Pseudomonas aeruginosa PAO1, E.coli K-12. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77111
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified