Vector Database
Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pRD8
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6300
- Notes
- The 5' 10 bp of U3 in the left LTR were deleted, so there is no homology between the two U3 sequences and the risk of recombination is reduced. [1] A replication-defective retroviral vector which is promoter-deficient in the right LTR and cannot express retroviral RNA. Foreign gene expression is regulated by the SV40 early promoter. [1] pJD220SvHy (ATCC 67393) and pRD8 (ATCC 67394) differ in the orientation of the SV40 promoter/hygromycin phosphotransferase B sequences. pRD8 has an additional 3' RNA processing sequence between the left U5 and the hygromycin sequences. [1] Removal of SNV map units 7.747 to 8.127 results in deletion of the entire U3 sequence, except for 10 bp ath the 5' end containing the attL site. [1] Constructed from pJD220Hy by inserting a 3' processing sequence between SstI and SalI sites, removing the hygro gene, and replacing it in the opposite orientation with the SV40 promoter. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.6; EcoRI--2.8, 2.0, 1.5; HindIII--uncut; SalI--6.2; XhoI--6.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, vertebrate cells, D17 cell line for infection, D17-C3 helper cell line for transfection. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 67394
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified