Vector Database

The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - 2 micron ori. (personal communication) YE-type shuttle vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. (personal communication) pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). Constructed from pRS306 (ATCC 77141) by inserting a 1.891 kb fragment from the 2 micron plasmid into the AatII site and inserting BglII linkers into the SmaI site of the polylinker. (personal communication) Restriction digests of the clone give the following sizes (kb): PvuI--3.7, 1.9, 0.7; HindIII--6.3; EcoRV--4.8, 1.5; XbaI--3.5, 2.7; PvuII--5.9, 0.4. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)