Vector Database

One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. The order of the major features in this plasmid is:LEU2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla. YI-type integrating shuttle vector permitting visual detection of recombinants & production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (2.235 kb) containing the LEU2 gene was inserted into the NdeI site of pRSS56. All ends were blunted. Restriction digests of the clone give the following sizes (kb): HindIII--5.6; PvuII--5.1, 0.46; KpnI--3.8, 1.7; EcoRI--3.5, 2.0. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416306 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS304, pRS303, pRS306. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)