Vector Database

One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: URA3 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla. YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.112 kb) containing the URA3 gene was inserted into the NdeI site of pRSS56. All ends were blunted. Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.65; PvuI--3.9, 0.40; EcoRI--4.4; HindIII--4.4. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416307 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS304, pRS305, pRS303. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)