Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pRSVlacZII
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8900
- Notes
- The order of the major features in pRc/RSV is: RSV LTR - MCS - bovine growth hormone polyadenylation signal - M13 ori - SV40 early promoter - neoR - SV40 polyadenylation signal - pMB1 ori - bla. [1] Shuttle reporter plasmid permitting visual detection of activity by histochemical staining. [1] Presence of beta-galactosidase activity is used to follow cell lineage in culture or in situ. [1] pRSVlacZII (ATCC 77129), pRSVPAP (ATCC 77130) and pRSVADH (ATCC 77131) provide distinct color-staining reactions (aqua blue, red, and blue-black respectively) to permit simultaneous analysis of multiple lineages. [1] Constructed by cloning the 3.9 kb HindIII/BamHI fragment from pM-MuLV-SV-lacZ into pRc/RSV. [1] Transient expression experiments should be performed to examine the RSV promoter efficiency in different cell lines. The RSV promoter can be replaced by excising with a BglII/HindIII digest. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 3.4, 1.2; HindIII--9.0; BglII--9.0, KpnI--4.5 (doublet); XbaI--9.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, vertebrate cells, E.coli. Related vectors: pRcRSV from Invitrogen, pM-MuLV-SV-lacZ, pRSVADH, pRSVPAP. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77129
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified