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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Created by Moore, July 1995, under contract with NCBI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.85; ClaI--2.85. (ATCC staff) One of a series of plasmid expression vectors (ATCC 37677 to 37679) representing multiple reading frames for expression from trpE promoter. The fusion proteins produced contain less than 25 amino acids of the trpE protein. This vector contains the trpE operon and 51 bp of the trpE gene, followed by a 10-mer EcoRI linker and the M13mp13 multiple cloning site (MCS). There are stop codons after th MCS XbaI and ClaI sites, in frame from the GAA of the EcoRI site. When E.coli CAG-456 (a protease deficient host) is used this vector yields large amounts (100 mg/liter or 45% of the soluble protein) of the fusion protein. Use E.coli SG935 (ATCC 39623), SG936 (ATCC 39624) or WM6 (ATCC 47020) as host for high level production of recombinant fusion protein. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli CAG-456, E.coli DH1. Related vectors: pATH1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
|GenBank:||M28835, M28836, M28837|