Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pS1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 2770
- Notes
- Created by Moore, July 1995, under contract with NCBI. Deposited by: J. Justin McCormick Cloning vector with the multiple cloning site expanded by inserting an oligonucleotide at the SmaI site of pUC19, thereby generating an amber mutation in the lacZ' coding sequence. [1] The plasmid contains the following restriction sites (bp from 0): DrdI--91, 992; KasI--233; PvuI--276, 2150; EcoRI--396; NotI--418; NsiI--493; HindIII--531; SapI--767; BsaI--1850; SspI--2585. (personal communication) The order of the major features in this plasmid is: KasI - 3' lacZ'amb1 - EcoRI/MCS/HindIII - 5' lacZ'amb1 - SapI - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): PvuI--1.9, 0.9; EcoRI--2.8; HindIII--2.8; NotI--2.8; SspI--2.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, E.coli JM109. Related vectors: pUC19. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77381
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified