Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSE1076
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9000
- Notes
- A derivative of pNKY51 with kanR from Tn903 inserted at the SmaI site of pNKY51. (personal communication) The order of the major features in this plasmid is: BglII (nt 1) - hisG - nt 1150 - URA3 - nt 2350 - kanR - nt 3850 - hisG - BamHI (nt 5000) - pMB1 ori - ampR. (personal communication) Restriction digests of the clone give the following sizes (kb): BglII--9.0; BamHI--9.0; BamHI/BglII--5.2, 3.8. (ATCC staff) A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains. Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, & selecting for stable integrants. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77271
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified