Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSHT
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4000
- Notes
- Deposited by: Phillip Bird Shuttle expression vector providing a start codon and signal sequence (from influenza virus hemagglutinin HA) necessary to express membrane and secretory proteins in mammalian cells. [1] Contains stop codons in all three frames after cloning sites. [1] The order of the major features in the phagemid is: pMB1 ori - ampR - M13 ori - ClaI - SV40 ori - SV40 early promoter - T7 promoter - HindIII - HA signal sequence - BamHI/SmaI/SpeI - termination signals - EcoRI - NheI- SV40 intron - BclI - SV40 polyadenylation. [1] Restriction digests of the clone give the following sizes (kb): SmaI--4.0; BamHI--4.0; HindIII--4.0. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101, vertebrate cells. (Information source: <a href=http://seq.yeastgenome.org/vectordb target=_blank>VectorDB</a>.)
- Catalog Number
- 77372
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified